RESEARCH ASSOCIATE I - PROTEOMICS

Sanford Burnham Prebys is much more than a research facility and hub for innovation; it’s a vibrant global community of talented scientists and researchers from over 30 countries. This worldwide representation enriches our perspectives and enhances our creative approach to solving complex scientific challenges.

We strive to be leaders not only in biomedical research but also in creating an environment where excellence thrives through collaboration across cultures and backgrounds. Our mission is to advance the biomedical sciences by cultivating the next generation of scientific leaders, providing meaningful opportunities for researchers of all backgrounds to learn, innovate, and make breakthrough discoveries that improve human health.

Together, we translate science into health.

The duties and responsibilities contained in the job description are intended to be examples of the accountabilities for which the person in the position will demonstrate competency through performance. The job description is not intended to be an all-inclusive list. Duties and responsibilities are subject to change and other duties may be assigned as necessary.

Position Summary:

The Sanford Burnham Prebys Medical Discovery Institute (SBP) is seeking a Research Associate I to join its state-of-the-art Proteomics Core Facility. We are looking for a highly motivated and detail-oriented individual to perform high-quality sample preparation for downstream mass spectrometry-based proteomics analyses.

Working under the supervision of the Core Director, the Research Associate I will collaborate closely with Proteomics Specialists and other Core staff to process a wide variety of biological samples using established and emerging proteomics workflows. Primary responsibilities include protein extraction, digestion, enrichment, and other sample preparation techniques, as well as operation of automated liquid handling platforms to support high-throughput workflows. The successful candidate will help ensure the consistent delivery of high-quality samples for downstream LC-MS/MS analysis while maintaining rigorous quality standards and laboratory organization.

The Proteomics Core Facility is equipped with advanced automation systems, including an Agilent AssayMap Bravo, SEER SP100 platform, chromatography systems, and Thermo Fisher Orbitrap mass spectrometers (Orbitrap Fusion Lumos, Q Exactive, Orbitrap Elite, and Quantiva Triple Quadrupole). This position provides an opportunity to contribute to cutting-edge biomedical research by supporting investigators across diverse scientific disciplines in both academic and industry collaborations. Opportunities for professional development, technical training, and co-authorship on collaborative publications are available.

The successful candidate must be organized, adaptable, and able to manage multiple projects in a fast-paced, service-oriented environment while maintaining meticulous attention to detail and a commitment to reproducible, high-quality results.

Duties and Responsibilities:

• Perform sample preparation for mass spectrometry-based proteomics analyses, including protein extraction, quantification, reduction/alkylation, enzymatic digestion, peptide cleanup, fractionation, enrichment, and other established workflows.
• Operate and maintain automated liquid handling systems (e.g., Agilent AssayMap Bravo, SEER SP100, and other laboratory automation platforms) following established standard operating procedures.
• Prepare reagents, buffers, standards, and quality control samples, ensuring accuracy, consistency, and reproducibility across projects.
• Maintain detailed experimental records, sample tracking, and laboratory documentation in accordance with Core Facility policies and quality standards.
• Perform routine quality control procedures and assist with troubleshooting sample preparation workflows to ensure high-quality downstream mass spectrometry analyses.
• Assist with the routine maintenance, calibration, and organization of laboratory equipment and workspaces; monitor laboratory supplies and coordinate inventory and reagent replenishment.
• Collaborate closely with Proteomics Specialists and Core Facility staff to support diverse research projects from academic and industry investigators.
• Contribute to the development, optimization, and validation of new sample preparation workflows and automation protocols as needed.
• Assist with data organization, preparation of technical summaries, and documentation of experimental results.
• Adhere to institutional safety guidelines, laboratory best practices, and established standard operating procedures.
• Perform other duties as assigned to support the mission and operations of the Proteomics Core Facility.

Minimum Qualifications:

Education: Bachelor's degree in biology, biochemistry, molecular biology, chemistry, or a related life science field is required.

Experience: One (1) to three (3) years of relevant laboratory experience in biochemistry, molecular biology, proteomics, or a related discipline; or an equivalent combination of education, training, and/or experience from which comparable knowledge, skills, and abilities have been attained.

Knowledge, Skills, and Abilities:

• Experience with protein sample preparation techniques and standard biochemical laboratory methods.
• Working knowledge of techniques such as protein extraction, protein quantification, enzymatic digestion, immunoprecipitation, affinity purification, SDS-PAGE, Western blotting, and protein purification.
• Experience following standard operating procedures (SOPs) with strong attention to detail and reproducibility.
• Ability to maintain accurate laboratory records and manage multiple projects in a fast-paced environment.
• Strong organizational, analytical, and problem-solving skills.
• Excellent verbal and written communication skills and the ability to work collaboratively in a team-oriented, service-focused environment.
• Demonstrated willingness to learn new laboratory techniques and adapt to evolving technologies.

Certifications, Licenses, etc.: None required.

Other Knowledge, Skills and/or Abilities:

• Strong attention to detail and commitment to producing accurate, reproducible, and high-quality experimental results.
• Excellent organizational and time management skills, with the ability to prioritize and manage multiple projects simultaneously.
• Ability to work independently with minimal supervision while contributing effectively as part of a collaborative, service-oriented team.
• Strong troubleshooting and problem-solving skills, with the ability to identify and resolve routine technical issues.
• Excellent interpersonal, verbal, and written communication skills, with the ability to interact professionally with investigators, collaborators, and Core Facility staff.
• Demonstrated commitment to maintaining accurate laboratory documentation and following established standard operating procedures (SOPs).
• Ability to learn and apply new laboratory techniques, instrumentation, and automation technologies.
• Proficiency with Microsoft Office applications (Word, Excel, PowerPoint) and electronic record-keeping systems.
• Ability to maintain a safe, organized, and efficient laboratory environment while adhering to institutional safety policies and regulations.
• Commitment to providing high-quality scientific support and customer service to a diverse research community.
• Preferred but not required: Familiarity with mass spectrometry-based proteomics workflows, including LC-MS/MS sample preparation, quantitative proteomics (e.g., TMT or label-free), automated liquid handling systems, Orbitrap mass spectrometry platforms, or proteomics data analysis software (e.g., Proteome Discoverer, MaxQuant, Skyline).

Supervisory Responsibilities:

Direct: None

Indirect: None

Work Environment:

The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job.

The laboratory is a controlled environment designed to support various scientific activities. It is equipped with specialized tools and equipment, including but not limited to microscopes, centrifuges, and incubators, ensuring precision and safety during experiments. The space adheres to strict safety protocols, with personal protective equipment (PPE) provided and mandatory for all personnel.

The atmosphere is typically collaborative, fostering teamwork and communication among researchers. Workstations are organized for efficiency, with designated areas for specific tasks such as sample preparation, analysis, and data management. The laboratory maintains a clean and orderly space, emphasizing adherence to hygiene and safety standards.

Regular training sessions and meetings are conducted to keep staff updated on best practices and new technologies. Overall, the laboratory environment is dynamic, requiring adaptability and attention to detail, with a strong focus on innovation and research excellence.

Physical Requirements:

The role in the laboratory may require specific physical capabilities, including:

• Standing and Walking: Ability to stand for extended periods and move between different workstations.
• Lifting and Carrying: Capability to lift and carry equipment and materials, typically up to 25 pounds, with occasional heavier items.
• Manual Dexterity: Proficiency in using fine motor skills for handling delicate instruments and samples.
• Repetitive Motions: Comfort with repetitive tasks, such as pipetting or using laboratory machinery.
• Visual Acuity: Ability to distinguish colors and details in specimens and data.
• Bending and Stooping: Flexibility to reach and access equipment or samples stored at various heights.

The physical demands may vary depending on the specific laboratory function, but overall, a moderate level of physical fitness and dexterity is essential for effective performance.

Work Location: This is an onsite position; candidates must be willing to work onsite in San Diego. Out-of-state applicants must disclose their willingness to relocate.

Compensation: The expected hiring range for this position is $22.22-$30.06/hourly commensurate with experience.

We welcome talented individuals of all backgrounds regardless of gender, sex, religion, race, national origin, citizenship, age, disability, perceived disability, pregnancy, pregnancy-related condition, reproductive health decisions, sexual orientation, gender identity, gender expression, genetic information, HIV/AIDS, marital status, covered veteran status, or any other characteristic protected by local, state, or federal laws, rules, or regulations. We are proud to be an equal employment opportunity employer.

As part of this commitment, Sanford Burnham Prebys Medical Discovery Institute will ensure that persons with disabilities are provided reasonable accommodations for the hiring process. If a reasonable accommodation is needed, please contact our Benefits Team at (858) 646-3100 or via e-mail at benefits@sbpdiscovery.org