University of Pittsburgh
Pittsburgh, PA
Laboratory Research Technician UPCI Research Lab-Nacev, Benjamin - Pennsylvania-Pittsburgh - (26004399) Performs a range of molecular biology, cell biology, epigenomic, and in vivo laboratory procedures in support of a translational sarcoma research program focused on ATRX-deficient soft tissue sarcomas (Nacev Laboratory, Division of Hematology/Oncology, UPMC Hillman Cancer Center). Core duties include maintaining and manipulating human and murine sarcoma cell lines, performing drug treatment and cell viability assays; immunoblotting; chromatin and epigenomic profiling (CUT&RUN, ATAC-seq); nucleic acid extraction and next-generation sequencing library preparation (RNA-seq, whole-genome bisulfite sequencing); and murine allograft tumor studies conducted under an approved IACUC protocol. The technician also supports general laboratory operations, including supply management, equipment maintenance, and accurate, timely experimental record keeping in accordance...