4 laboratory research technician iii jobs found in Pittsburgh, PA

Laboratory Research Technician III Med-Medicine - Pennsylvania-Pittsburgh - (26003190) The Pulmonary, Allergy, Critical Care and Sleep Medicine Division of the DOM. The division is made of up approximately 70 clinical staff and over 100 research staff who work in a variety of locations including: Bridgeside Point, Biomedical Science Tower (BST), UPMC Hospitals including Presbyterian, Shadyside, East, Jameson and Mercy. The division provides world class services across 10 inpatient services at five hospitals and over 70,000 outpatient office visits. We have a major research mission that focuses on basic, clinical, and translational research on the lung, microbiome, COVID-19, sleep medicine, and allergy and immunology. The laboratory tech will be responsible for performing cell culture, processing lung tissue for western blotting, PCR, and immunostating. They will develop protocols for new assays, and will analyze, interpret, and summarize results from lab...

Laboratory Research Technician III Med-Surgery - Pennsylvania-Pittsburgh - (26002831) The Department of Surgery seeks to establish a new Next Generation Sequencing (NGS) Technician position to support the growing scope and complexity of its genomics and humansample–driven research programs. This role will provide technical expertise in genomics workflows including qPCR, singlecell work, singlecell library creation, nextgeneration sequencing library preparation, and data quality control, as well as upstream processes such as PBMC and plasma isolation. The NGS Technician will support multiple investigators and collaborative projects, manage and track human sample inventories, and coordinate with institutional resources such as MACRO to secure human biospecimens in compliance with approved protocols. By standardizing best practices, improving data quality and reproducibility, and strengthening operational capacity, this position will play a critical role in...

Laboratory Research Technician IV Talent Acquisition - Pennsylvania-Pittsburgh - (26003389) Job Summary Laboratory research technician iv The University of Pittsburgh School of Medicine seeks a Laboratory Research Technician IV to work in Pittsburgh, PA. Duties include designing/performing: (i) isolation, activation, and expansion of T cells (ii) cryopreservation, sub-culturing, and revival of various cell lines and primary cells; (iii) cell-based assays including cytotoxicity, cell viability, and cell proliferation assays; (iv) immunofluorescence imaging using confocal microscopy and single-cell time-lapse imaging; (v) isolation and handling of PBMCs from human whole blood; (vi) multicolor flow cytometry, SDS-PAGE, western blot, multi-mode reader-based assays and various biochemical assays; and (vii) data analysis using Graphpad Prism, FlowJo, Excel and Image-J. Must have a master’s degree (or foreign equivalent degree) in Biology,...

Research Technician (Manni Lab) Med-Pharmacology and Chemical Biology - Pennsylvania-Pittsburgh - (26003305) Research Technician III position is available immediately in the Department of Pharmacology and Chemical Biology, University of Pittsburgh, to provide research support in a lung immunology focused laboratory. This is a staff position equivalent to a lab technician. The candidate will oversee daily lab operations, maintain mouse colonies, perform routine lab procedures, and train junior fellows and students. The salary will be commensurate with skills and experience. Key Responsibilities: • Manage and maintain mouse colonies, generate genetically modified mouse models, and conduct animal experiments. • Perform independent laboratory experiments, including developing experimental procedures and preparing reagents and specimens. • Design and execution of extractions from different samples, including blood, airway epithelium,...